Inhibition of sucrose phosphatase by sucrose
نویسندگان
چکیده
منابع مشابه
Specific Sucrose Phosphatase from Plant Tissues
1. A phosphatase that hydrolyses sucrose phosphate (phosphorylated at the 6-position of fructose) was isolated from sugar-cane stem and carrot roots. With partially purified preparations fructose 6-phosphate, glucose 6-phosphate, fructose 1-phosphate, glucose 1-phosphate and fructose 1,6-diphosphate are hydrolysed at between 0 and 2% of the rate for sucrose phosphate. 2. The activity of the enz...
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Sucrose-phosphatase (SPP) catalyzes the final step in the pathway of sucrose biosynthesis in both plants and cyanobacteria, and the SPPs from these two groups of organisms are closely related. We have crystallized the enzyme from the cyanobacterium Synechocystis sp PCC 6803 and determined its crystal structure alone and in complex with various ligands. The protein consists of a core domain cont...
متن کاملRegulation of sucrose-sucrose-fructosyltransferase in barley leaves.
The activity of sucrose-sucrose-fructosyltransferase (SST), a vacuolar enzyme strongly induced by light in excised leaves of barley (Hordeum vulgare L.), rapidly declined even in continuous light upon feeding of cycloheximide (CHI). The rate of decline was similar to that observed in light-treated leaves that were placed into darkness, in the presence or absence of CHI. The protease inhibitor l...
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Bioinformatic analysis indicates that sucrose phosphate synthase (SPS) contains a putative C-terminal sucrose phosphate phosphatase (SPP)-like domain that may facilitates the binding of SPP. If an SPS-SPP enzyme complex exists, it may provide sucrose biosynthesis with an additional level of regulation, forming a direct metabolic channel for sucrose-6-phosphate between these two enzymes. Herein,...
متن کاملSubstrate Inhibition of Maize Endosperm Sucrose Synthase by Fructose and Its Interaction with Glucose Inhibition*
Sucrose synthase (EC 2.4.1.13) was purified to homogeneity from developing maize lZea mays L.) endosperm. Substrate saturation and inhibitor kinetics were examined for the sucrose synthase reaction. The Km-values for fructose and uridine diphosphate glucose (UDPGlc) were estimated to be 7.8 mM and 76 IlM, respectively. Fructose concentrations over 20 mM inhibited sucrose synthase in an uncompet...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1967
ISSN: 0006-2936
DOI: 10.1042/bj1020401